Immunocytochemical Localization of Glutamine Synthetase in Organs of Phaseolus vulgaris L.

Heterogeneity of Glutamine Synthetase Polypeptides in Phaseolus vulgaris L.

Glutamine synthetases from roots, nodules, and leaves of Phaseolus vulgaris L. have been purified to homogeneity and their polypeptide composition determined.The leaf enzyme is composed of six polypeptides. The cytosolic fraction contains two 43,000 dalton polypeptides and the chloroplastic enzyme is formed by four 45,000 dalton polypeptides. Root glutamine synthetase consists only of the same ...

Two glutamine synthetase genes from Phaseolus vulgaris L. display contrasting developmental and spatial patterns of expression in transgenic Lotus corniculatus plants.

The gln-gamma gene, which specifies the gamma subunit of glutamine synthetase in Phaseolus vulgaris L., has been isolated and the regulatory properties of its promoter region analyzed in transgenic Lotus corniculatus plants. A 2-kilobase fragment from the 5'-flanking region of gln-gamma conferred a strongly nodule-enhanced pattern of expression on the beta-glucuronidase reporter gene. Parallel ...

Proline metabolism in response to highest nitrogen dosages in green bean plants (Phaseolus vulgaris L. cv. Strike)

The objective of the present work was to determine what impact extremely high nitrogen dosages would have on proline metabolism in order to use this amino acid as a bioindicator of N status of green bean plants (Phaseolus vulgaris L. cv. Strike). In this effort, we identified the most favourable pathway of proline synthesis under our experimental conditions. The N was applied to the nutrient so...

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.

Effect of Asparagine or Glutamine on Growth

ffects of L-asparagine and L-glutamine on growth, pigments content and metabolism of haseolus vulgaris plants, at two stages of plant growth and development in was investigated in icropropagated tissues (in vitro). In general, all growth parameters increased by 1 & 2 mM sparagine or glutamine treatments while decreased in response to other concentrations (3, 4 & 5 M), Similarly total carbohydra...